Genetic and Functional Assessment of the Role of the rs13431652-A and rs573225-A Alleles in the G6PC2 Promoter that Strongly Associate With Elevated Fasting Glucose Levels
نویسندگان
چکیده
Nabila Bouatia-Naji 1,2* , Amélie Bonnefond 1,2* , Devin A. Baerenwald 3 , Marion Marchand 1,2 , Marco Bugliani 4 , Piero Marchetti 4 , François Pattou 5 , Richard L. Printz 3 , Brian P. Flemming 3 , Obi C. Umunakwe 3 , Nicholas L. Conley 3 , Martine Vaxillaire 1,2 , Olivier Lantieri 6 , Beverley Balkau 7 , Michel Marre 8 , Claire Lévy-Marchal 9 , Paul Elliott 10 , Marjo-Riitta Jarvelin 10,11 , David Meyre 1,2 , Christian Dina 1,2# , James K. Oeser 3 , Philippe Froguel 1,2,12 and Richard M. O’Brien 3 ,
منابع مشابه
Genetic and Functional Assessment of the Role of the rs13431652-A and rs573225-A Alleles in the G6PC2 Promoter That Are Strongly Associated With Elevated Fasting Glucose Levels
OBJECTIVE Genome-wide association studies have identified a single nucleotide polymorphism (SNP), rs560887, located in a G6PC2 intron that is highly correlated with variations in fasting plasma glucose (FPG). G6PC2 encodes an islet-specific glucose-6-phosphatase catalytic subunit. This study examines the contribution of two G6PC2 promoter SNPs, rs13431652 and rs573225, to the association signal...
متن کاملGenetic and Functional Assessment of the Role of the rs13431652-A and rs573225-A Alleles in the G6PC2 Promoter that Strongly Associate With Elevated Fasting Glucose Levels
Genotype/expression correlation in human pancreatic cDNAs. Isolated pancreatic islets were prepared by collagenase digestion and density gradient purification (1; 2). After isolation, islets were cultured free-floating in M199 culture medium (Sigma-Aldrich) at 5.5 mmol/l glucose concentration. Within 3 days of isolation, the islets were used for RNA extraction. Total RNA was extracted from the ...
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OBJECTIVE The G6PC2 gene encoding islet-specific glucose-6-phosphatase related protein (IGRP) has a common promoter variant, rs573225 (-231G/A), located within a Foxa binding site. We tested the cis-regulatory effects of rs573225 on promoter activity and its association with insulin response to oral glucose. RESEARCH DESIGN AND METHODS Functional effects of rs573225 were explored in transfect...
متن کاملMeta-analyses of the association of G6PC2 allele variants with elevated fasting glucose and type 2 diabetes
OBJECTIVE To collectively evaluate the association of glucose-6-phosphatase catalytic unit 2 (G6PC2) allele variants with elevated fasting glucose (FG) and type 2 diabetes (T2D). DESIGN Meta-analysis. DATA SOURCES PubMed, Web of Knowledge and Embase databases. STUDY SELECTION Full text articles of studies that identified an association of G6PC2 with T2D and elevated FG. PATIENT INVOLVEM...
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Introduction: Ghrelin, a gut neuropeptide, plays an important role in appetite, food intake and weight regulation. The aim of the present study was to determine serum ghrelin in relation to fasting glucose concentration and effective markers in glucose level (insulin, insulin resistance, beta- cell function) in none-diabetic obese males. Methods: We studied the baseline glucose, serum insulin a...
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